Glossary entry (derived from question below)
Portuguese term or phrase:
com a visualização da expressão
English translation:
expression [of peptides] was observed
Added to glossary by
liz askew
Nov 17, 2011 18:18
12 yrs ago
Portuguese term
com a visualização da expressão
Portuguese to English
Other
Biology (-tech,-chem,micro-)
A expressão da proteína foi verificada em gel SDS-PAGE 15% corado com Coomassie Brilliant Blue com a visualização da expressão de peptídeos de massa molecular de aproximadamente 19KDa, fusionado a resíduos de histidina (His-Tag).
Proposed translations
(English)
3 +1 | expression [of peptides] were observed | liz askew |
5 | peptides were visualized | Christina Paiva |
Change log
Dec 1, 2011 19:16: liz askew Created KOG entry
Proposed translations
+1
1 hr
Selected
expression [of peptides] were observed
I think you need to change the sentence round a bit
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Note added at 1 hr (2011-11-17 19:35:54 GMT)
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www.patents.com/us-20090215113.html - Translate this page
... Tris/glycine SDS-PAGE and subsequent Coomassie Brilliant Blue staining. ..... G. Peptide Production By Bacterial Expression [0097]The peptides which .... Mercaptoethanol, 12.5% Glycerol, 0.005% Bromphenol Blue) to elute bound PT. ..... Ala Ser Ala Ile 50206PRTArtificial SequenceSynthetic histidine tag 20His His ...
of course that should be
expression.......................WAS observed
depends on how you parse the English
--------------------------------------------------
Note added at 1 hr (2011-11-17 19:35:54 GMT)
--------------------------------------------------
www.patents.com/us-20090215113.html - Translate this page
... Tris/glycine SDS-PAGE and subsequent Coomassie Brilliant Blue staining. ..... G. Peptide Production By Bacterial Expression [0097]The peptides which .... Mercaptoethanol, 12.5% Glycerol, 0.005% Bromphenol Blue) to elute bound PT. ..... Ala Ser Ala Ile 50206PRTArtificial SequenceSynthetic histidine tag 20His His ...
of course that should be
expression.......................WAS observed
depends on how you parse the English
3 KudoZ points awarded for this answer.
5 hrs
peptides were visualized
Detergent binding explains anomalous SDS-PAGE migration of ...
www.pnas.org/content/106/6/1760.fullby A Rath - 2009 - Cited by 77 - Related articles
A further proviso for MW estimation by SDS-PAGE is a consistent amount of ... **Peptide segments** that contain an abundance of hydrophobic ..... ...TM3/4 hairpins [uma proteína mutante] were expressed and purified as previously described (13). ...
um trecho do artigo acima:
**Proteins were visualized** with Coomassie Blue staining. Hairpin apparent MW values were estimated from the migration rates of Mark-12 markers (Invitrogen), ..
Em eletroforese com gel de poliacrilamida aka PAGE/SDS apenas os peptídeos/proteínas são visualizados como resultado da expressão do gene/genes estudado(s)... ou seja - não se visualiza a expressão, mas seu resultado.
HTH
www.pnas.org/content/106/6/1760.fullby A Rath - 2009 - Cited by 77 - Related articles
A further proviso for MW estimation by SDS-PAGE is a consistent amount of ... **Peptide segments** that contain an abundance of hydrophobic ..... ...TM3/4 hairpins [uma proteína mutante] were expressed and purified as previously described (13). ...
um trecho do artigo acima:
**Proteins were visualized** with Coomassie Blue staining. Hairpin apparent MW values were estimated from the migration rates of Mark-12 markers (Invitrogen), ..
Em eletroforese com gel de poliacrilamida aka PAGE/SDS apenas os peptídeos/proteínas são visualizados como resultado da expressão do gene/genes estudado(s)... ou seja - não se visualiza a expressão, mas seu resultado.
HTH
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